Conversion of D-xylose to D-xylulose in extracts of Lactobacillus pentosus.
نویسندگان
چکیده
منابع مشابه
The role of xylulose 5-phosphate in xylose metabolism of Lactobacillus pentosus.
Lampen (1) observed that cell-free extracts of LactobaciZZus pentosus grown on D-xylose catalyzed the disappearance of n-xylose in the presence of ATP.’ The phosphate esters which accumulated were identified as a mixture of D-ribose 5-phosphate and D-ribulose 5-phosphate. Neither n-xylose 5-phosphate nor D-xylulose 5-phosphate was found in the reaction mixture. Mitsuhashi and Lampen (2) also de...
متن کاملComplementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.
The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection marker for Lactobacillus spp.
متن کاملMolecular cloning and functional expression in lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis.
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H+ symporters of E. coli, XylE (57%), and Bacillus ...
متن کاملFunctional expression in Lactobacillus plantarum of xylP encoding the isoprimeverose transporter of Lactobacillus pentosus.
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown...
متن کاملTransport of D-xylose in Lactobacillus pentosus, Lactobacillus casei, and Lactobacillus plantarum: evidence for a mechanism of facilitated diffusion via the phosphoenolpyruvate:mannose phosphotransferase system.
We have identified and characterized the D-xylose transport system of Lactobacillus pentosus. Uptake of D-xylose was not driven by the proton motive force generated by malolactic fermentation and required D-xylose metabolism. The kinetics of D-xylose transport were indicative of a low-affinity facilitated-diffusion system with an apparent K(m) of 8.5 mM and a V(max) of 23 nmol min(-1) mg of dry...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 204 2 شماره
صفحات -
تاریخ انتشار 1953